Most 45 Colt cylinder throats are opened to .4525". Many jacketed bullets are made at .452". Most of the old timers cast their Colt, or Colt clone bullets from a .454 mold and sized at .454 or .453". Some of the newer guns shoot cast sized at .452" just fine. My Vaquero does.
Size of cylinder throat does make a difference. I have a DW .357 that I used in competition. Every once in a while I would get a flier. One day I marked each cylinder with a marker and shot once at each of 6 targets. I went through 5 times and compared the 5 shot groups. It was easy to find the cylinder that was spitting the fliers. I marked that cylinder with black sharpie, and then went through again. I wanted to confirm EXACTLY where point of impact was in relationship to center of target for each of the other cylinders, and as the gun got lighter. It was surprising how much that gun moved as it got lighter 220 grains at a time.
Hope this helps.
Steve